Abstract

Cells rely on endocytosis to perform a myriad of cellular functions. For the foreseeable future this multifaceted process will become increasingly complex as new pathways are discovered and novel (and old) proteins are revealed to orchestrate endocytic events. Within the field of drug delivery there is, most often, a reliance on endocytosis to deliver macromolecular therapies to cells and intracellular compartments. There include genes, peptides and proteins and the success of this strategy lies not only in designing new drug delivery systems (DDS) but also on gaining an improved understanding on how they enter cells and are then processed on the endolysosomal system.

Our laboratory together with national and international collaborators have been studying endocytic pathways with a view to elucidating a role they may have in uptake of ligands, receptors and DDS, and how they traffic these entities towards their ultimate fate.  In this seminar I will summarize recently published and unpublished observations describing how endocytosis can be significantly enhanced via ligand-mediated clustering of receptors on the plasma membrane.  This also leads to differences in intracellular traffic and subcellular fate that may have profound effects on intracellular targeting of therapeutics. These include ligand decorated nanoparticles, antibodies or antibody drug conjugates and could also extend to cell penetrating peptides (CPPs). These peptides have been suggested to gain access to cells via clustering of sugars and lipids and processes that rely on the actin cytoskeleton. Recent data further investigating the role of actin on uptake of the cationic CPPs attached to fluorophores and proteins will also be discussed.

Kontakt information

Prof. Arwyn Tomos Jones
Professor of  Membrane Traffic and Drug Delivery
Cardiff School of Pharmacy and Pharmaceutical Sciences
Redwood Building, Cardiff University, Cardiff, Wales CF10 3NB

Telefon:  +44 (0)29 2087 6431
E-post: jonesAT@cardiff.ac.uk

Hemsida

 

Värd

Professor Ülo Langel, Institutionen för neurokemi