Abstract

RNA interference (RNAi) is a powerful tool to inhibit the expression of a target gene. We employ the technology for several medically relevant fields. One approach is to inhibit potential pain receptors for target validation. Different application methods will be compared. In addition, we use RNAi to inhibit the heart pathogenic coxsackievirus B3 (CVB-3). Adeno-associated viruses (AAV) have been proven to be powerful tools for the delivery of transgenes to the heart. Self-complementary, pseudotyped AAV vectors can be used to transfer short hairpin RNA (shRNA) expression cassettes to cardiomyocytes. This approach was successfully employed to treat an acute heart infection with CVB‑3 in a mouse model for virus-induced myocarditis. More recently, we developed 3D ex vivo organ models for infection studies. Our initial experiments demonstrate that a decellularized rat liver can be repopulated with human hepatic cells. This model can be used to study viral vectors for gene delivery and will be employed for the development of new antiviral strategies.

Kontaktinformation

Prof. Dr. Jens Kurreck
Technische Universität Berlin
Institute of Biotechnology, TIB 4/3-2
Department of Applied Biochemistry
Gustav-Meyer-Allee 25, 13355 Berlin, Germany

Tel.: +49 (0)30 314 27582
Fax:  +49 (0)30 314 27502
E-post: jens.kurreck@tu-berlin.de

Värd

Professor Ülo Langel, Institutionen för neurokemi